Eight- week- old female mice were inoculated on day 0 with IFV A influenza A PR 8 34 H1N1 originating from ATCC VR- 1469 via the intranasal route at 175 PFU mouse 50 ОјL, 1000 PFU mouse 50 ОјL, or 760 PFU mouse 50 ОјL, depending on the batch of the virus Supplemental Figure 4, A and B, and Supplemental Figure 5, A and C, under anesthesia by intraperitoneal injection of anesthetic 30 mg kg Zoletil 50 6 mg kg xylazine. is doxycycline used for